Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GACT)3
Start:498963 End:498974
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGGTAACGAAACGACCTTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTCTCGCGCCTTATGAAAA | |||||||||||||
| Tm_F_1 | 59.99 | Size_F_1 | 20 | Tm_R_1 | 59.982 | Size_R_1 | 20 | PRODUCT Size | 249 bp | Primer Start | 498815 | Primer End | 499063 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGGGTAACGAAACGACCTTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTCACTCTCGCGCCTTAT | |||||||||||||
| Tm_F_2 | 59.99 | Size_F_2 | 20 | Tm_R_2 | 60.118 | Size_R_2 | 20 | PRODUCT Size | 254 bp | Primer Start | 498815 | Primer End | 499068 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGGGTAACGAAACGACCTTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGCGACGTTGAATACAAGA | |||||||||||||
| Tm_F_3 | 59.99 | Size_F_3 | 20 | Tm_R_3 | 60.257 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 498815 | Primer End | 499091 |