Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GACA)3
Start:819481 End:819492
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTCAAAGGTGAAGCGGAGTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCTTCTGCTCCTCTCCTGA | |||||||||||||
| Tm_F_1 | 59.333 | Size_F_1 | 20 | Tm_R_1 | 60.214 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 819305 | Primer End | 819574 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCAGTCAAAGGTGAAGCGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTCCTTCTGCTCCTCTCCT | |||||||||||||
| Tm_F_2 | 60.255 | Size_F_2 | 20 | Tm_R_2 | 59.127 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 819301 | Primer End | 819576 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCAGTCAAAGGTGAAGCGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCCTTCTGCTCCTCTCCTG | |||||||||||||
| Tm_F_3 | 60.255 | Size_F_3 | 20 | Tm_R_3 | 59.127 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 819301 | Primer End | 819575 |