Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAATA)3
Start:610040 End:610054
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAACTCGTGCTCCGAAAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGAAATCAAGCATTGGGTCG | |||||||||||||
| Tm_F_1 | 59.993 | Size_F_1 | 20 | Tm_R_1 | 60.074 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 609944 | Primer End | 610217 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCCTTTCAACAACATACCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGAACTCGTCTTTGGTGTCA | |||||||||||||
| Tm_F_2 | 60.22 | Size_F_2 | 20 | Tm_R_2 | 59.873 | Size_R_2 | 20 | PRODUCT Size | 279 bp | Primer Start | 609921 | Primer End | 610199 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACAACATACCGGCCAAAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CATTGGGTCGAACTCGTCTT | |||||||||||||
| Tm_F_3 | 59.73 | Size_F_3 | 20 | Tm_R_3 | 60.111 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 609929 | Primer End | 610207 |