Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAAT)3
Start:148643 End:148654
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGAGAGGAAAGAAAAGCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTTGATCCTTCCCCTTTGA | |||||||||||||
| Tm_F_1 | 59.708 | Size_F_1 | 20 | Tm_R_1 | 59.6 | Size_R_1 | 20 | PRODUCT Size | 251 bp | Primer Start | 148527 | Primer End | 148777 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACGAACACGAACTGAGAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTCGCTCCTGGACTACTG | |||||||||||||
| Tm_F_2 | 59.461 | Size_F_2 | 20 | Tm_R_2 | 60.156 | Size_R_2 | 20 | PRODUCT Size | 260 bp | Primer Start | 148582 | Primer End | 148841 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACGAACACGAACTGAGAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGCTTCGCTCCTGGACTAC | |||||||||||||
| Tm_F_3 | 59.461 | Size_F_3 | 20 | Tm_R_3 | 60.156 | Size_R_3 | 20 | PRODUCT Size | 262 bp | Primer Start | 148582 | Primer End | 148843 |