Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAAC)3
Start:457510 End:457521
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCGCCTATCGGTAAAGCTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAAGGGAAACAGGAGGATGA | |||||||||||||
| Tm_F_1 | 59.832 | Size_F_1 | 20 | Tm_R_1 | 60.042 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 457409 | Primer End | 457635 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTAACCTCGGACGACACTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAAGGGAAACAGGAGGATGA | |||||||||||||
| Tm_F_2 | 60.171 | Size_F_2 | 20 | Tm_R_2 | 60.042 | Size_R_2 | 20 | PRODUCT Size | 163 bp | Primer Start | 457473 | Primer End | 457635 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAATTCCATTGCCGTAACCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAAGGGAAACAGGAGGATGA | |||||||||||||
| Tm_F_3 | 59.823 | Size_F_3 | 20 | Tm_R_3 | 60.042 | Size_R_3 | 20 | PRODUCT Size | 175 bp | Primer Start | 457461 | Primer End | 457635 |