Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAAAG)4
Start:226101 End:226120
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAAGGTCGCTTGCTTACTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AACCCCTGATCTGCTCCTTT | |||||||||||||
| Tm_F_1 | 60.162 | Size_F_1 | 20 | Tm_R_1 | 60.074 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 226070 | Primer End | 226341 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTCGAAAAATTGCTCCCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTGATAGAAGCTTGTCCCG | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 59.83 | Size_R_2 | 20 | PRODUCT Size | 171 bp | Primer Start | 226040 | Primer End | 226210 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTCGAAAAATTGCTCCCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGAATGCCTCTTTCTTGAC | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 59.813 | Size_R_3 | 20 | PRODUCT Size | 134 bp | Primer Start | 226040 | Primer End | 226173 |