Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAAA)4
Start:815938 End:815953
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGAAGCCAGACACTTTACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCCTTTCACTCCTTTCCCT | |||||||||||||
| Tm_F_1 | 59.882 | Size_F_1 | 20 | Tm_R_1 | 60.045 | Size_R_1 | 20 | PRODUCT Size | 169 bp | Primer Start | 815915 | Primer End | 816083 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCGAAGCCAGACACTTTACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTCCCTTTCACTCCTTTCCC | |||||||||||||
| Tm_F_2 | 59.882 | Size_F_2 | 20 | Tm_R_2 | 59.912 | Size_R_2 | 20 | PRODUCT Size | 170 bp | Primer Start | 815915 | Primer End | 816084 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCCGGCTAGTATCGAAAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCCTTTCACTCCTTTCCCT | |||||||||||||
| Tm_F_3 | 59.801 | Size_F_3 | 20 | Tm_R_3 | 60.045 | Size_R_3 | 20 | PRODUCT Size | 201 bp | Primer Start | 815883 | Primer End | 816083 |