Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAAA)4
Start:272238 End:272253
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACCTATCGGCCCCAGTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCTTTCCAACTCGGTTTCA | |||||||||||||
| Tm_F_1 | 60.173 | Size_F_1 | 20 | Tm_R_1 | 59.853 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 272020 | Primer End | 272286 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACCTATCGGCCCCAGTAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTTCCAACTCGGTTTCAC | |||||||||||||
| Tm_F_2 | 60.173 | Size_F_2 | 20 | Tm_R_2 | 59.717 | Size_R_2 | 20 | PRODUCT Size | 266 bp | Primer Start | 272020 | Primer End | 272285 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTAATGATTCCGTTGTGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTGCTGGACTGATTGAAGA | |||||||||||||
| Tm_F_3 | 60.478 | Size_F_3 | 20 | Tm_R_3 | 59.984 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 272148 | Primer End | 272425 |