Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAAA)3gacaagatgaaacaaaagaagaatgagactgaacaggaagggct(GAA)4aatgaaactagaattatccgtcacctatttcttctttcaatgaatgaaatcagggagacgaaagaaagacaagatgaaacgaaagatgaat(GA)6
Start:655061 End:655231
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTACCTGACCGGAACCAAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAGGTTCAGGTTCATTCGCT | |||||||||||||
| Tm_F_1 | 59.964 | Size_F_1 | 20 | Tm_R_1 | 60.255 | Size_R_1 | 20 | PRODUCT Size | 275 bp | Primer Start | 654991 | Primer End | 655265 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTACCTGACCGGAACCAAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTTCAGGTTCATTCGCTTC | |||||||||||||
| Tm_F_2 | 59.964 | Size_F_2 | 20 | Tm_R_2 | 59.676 | Size_R_2 | 20 | PRODUCT Size | 273 bp | Primer Start | 654991 | Primer End | 655263 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTACCTGACCGGAACCAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGGTTCAGGTTCATTCGCT | |||||||||||||
| Tm_F_3 | 59.288 | Size_F_3 | 20 | Tm_R_3 | 60.255 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 654990 | Primer End | 655265 |