Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAAA)3
Start:230852 End:230863
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGGAACAAAAACATGAGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCCTTCATTCAAACACCGT | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 21 | Tm_R_1 | 60.353 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 230658 | Primer End | 230927 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAAAGGAAAGACGAAACGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCCTTCATTCAAACACCGT | |||||||||||||
| Tm_F_2 | 59.847 | Size_F_2 | 21 | Tm_R_2 | 60.353 | Size_R_2 | 20 | PRODUCT Size | 124 bp | Primer Start | 230804 | Primer End | 230927 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGAAAGGAAAGACGAAACGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCCTTCATTCAAACACCGT | |||||||||||||
| Tm_F_3 | 59.847 | Size_F_3 | 21 | Tm_R_3 | 60.353 | Size_R_3 | 20 | PRODUCT Size | 125 bp | Primer Start | 230803 | Primer End | 230927 |