Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTTT)4acagtctggctgctgaatagtgtttag(AATC)3
Start:543314 End:543368
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCGGAGGGAAGTCAAGCTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCGGAGCAGACAGAAAGAAC | |||||||||||||
| Tm_F_1 | 59.948 | Size_F_1 | 20 | Tm_R_1 | 59.989 | Size_R_1 | 20 | PRODUCT Size | 134 bp | Primer Start | 543291 | Primer End | 543424 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCGGAGGGAAGTCAAGCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAGAGCAAGCAAAGCAAGC | |||||||||||||
| Tm_F_2 | 59.948 | Size_F_2 | 20 | Tm_R_2 | 59.911 | Size_R_2 | 20 | PRODUCT Size | 246 bp | Primer Start | 543291 | Primer End | 543536 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCGGAGGGAAGTCAAGCTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTTAAGCCAGCAAGCAGGA | |||||||||||||
| Tm_F_3 | 59.948 | Size_F_3 | 20 | Tm_R_3 | 60.154 | Size_R_3 | 20 | PRODUCT Size | 209 bp | Primer Start | 543291 | Primer End | 543499 |