Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTTT)4
Start:299783 End:299798
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGATCGAGACTTTCTTTGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGCTACCAAGCTGCTCCAC | |||||||||||||
| Tm_F_1 | 59.807 | Size_F_1 | 20 | Tm_R_1 | 60.164 | Size_R_1 | 20 | PRODUCT Size | 258 bp | Primer Start | 299670 | Primer End | 299927 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGATCGAGACTTTCTTTGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TATGAGCCTTGTGCGAGCTA | |||||||||||||
| Tm_F_2 | 59.807 | Size_F_2 | 20 | Tm_R_2 | 59.735 | Size_R_2 | 20 | PRODUCT Size | 272 bp | Primer Start | 299670 | Primer End | 299941 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGATCGAGACTTTCTTTGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGGTTATGAGCCTTGTGCG | |||||||||||||
| Tm_F_3 | 59.807 | Size_F_3 | 20 | Tm_R_3 | 60.27 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 299670 | Primer End | 299946 |