Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTTT)3acagtagagtaagtaaatgaatgacggaaccgtaaccgaacttatactgtactttctttgatttcaggtagagtcaag(ATTC)3
Start:642200 End:642301
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCATCCCCTCCCTTCTTAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGAGGAAGGAGCTCACACA | |||||||||||||
| Tm_F_1 | 60.25 | Size_F_1 | 20 | Tm_R_1 | 59.986 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 642093 | Primer End | 642359 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCATCCCCTCCCTTCTTAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAGGAAGGAGCTCACACAC | |||||||||||||
| Tm_F_2 | 60.25 | Size_F_2 | 20 | Tm_R_2 | 59.841 | Size_R_2 | 20 | PRODUCT Size | 266 bp | Primer Start | 642093 | Primer End | 642358 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCATCCCCTCCCTTCTTAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCAGGAAGGAGGAAGGAGC | |||||||||||||
| Tm_F_3 | 60.25 | Size_F_3 | 20 | Tm_R_3 | 59.778 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 642093 | Primer End | 642366 |