Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTTT)3
Start:330534 End:330545
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCAAGACTGACGATTCCGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCTGGTTGGATGTCATTTT | |||||||||||||
| Tm_F_1 | 59.874 | Size_F_1 | 20 | Tm_R_1 | 59.797 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 330318 | Primer End | 330585 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGACGATTCCGTGTAAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCTGGTTGGATGTCATTTT | |||||||||||||
| Tm_F_2 | 59.864 | Size_F_2 | 20 | Tm_R_2 | 59.797 | Size_R_2 | 20 | PRODUCT Size | 261 bp | Primer Start | 330325 | Primer End | 330585 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCAAGAAAGAAGCCTGCAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCTGGTTGGATGTCATTTT | |||||||||||||
| Tm_F_3 | 59.763 | Size_F_3 | 20 | Tm_R_3 | 59.797 | Size_R_3 | 20 | PRODUCT Size | 245 bp | Primer Start | 330341 | Primer End | 330585 |