Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTTA)3acctaatgggtctcgctcattgcttc(CCTG)3ccaccgcacgcaacctctctctatttcttagctttatttcagtgggtaaataggttgcttacttaattagacttctaacccaactccc(TACT)3
Start:532156 End:532305
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTGATCCTTGATTGCTTGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCAATCCGGCTAAAGAAAGA | |||||||||||||
| Tm_F_1 | 59.97 | Size_F_1 | 21 | Tm_R_1 | 59.433 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 532075 | Primer End | 532352 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTGATCCTTGATTGCTTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCAATCCGGCTAAAGAAAGA | |||||||||||||
| Tm_F_2 | 58.953 | Size_F_2 | 20 | Tm_R_2 | 59.433 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 532075 | Primer End | 532352 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTGATCCTTGATTGCTTGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGGCTAAAGAAAGAAGACG | |||||||||||||
| Tm_F_3 | 59.97 | Size_F_3 | 21 | Tm_R_3 | 59.108 | Size_R_3 | 20 | PRODUCT Size | 273 bp | Primer Start | 532075 | Primer End | 532347 |