Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTTA)3
Start:440451 End:440462
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTCGGCTACCTCTCCCACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAGGCCTTTAGGTGAGCTTG | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 20 | Tm_R_1 | 60.008 | Size_R_1 | 20 | PRODUCT Size | 229 bp | Primer Start | 440257 | Primer End | 440485 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAAGGTACGCTTTCTTTGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAGGCCTTTAGGTGAGCTTG | |||||||||||||
| Tm_F_2 | 59.883 | Size_F_2 | 20 | Tm_R_2 | 60.008 | Size_R_2 | 20 | PRODUCT Size | 162 bp | Primer Start | 440324 | Primer End | 440485 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTCGTAAATTTCCTGCGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGGCCTTTAGGTGAGCTTG | |||||||||||||
| Tm_F_3 | 59.853 | Size_F_3 | 20 | Tm_R_3 | 60.008 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 440215 | Primer End | 440485 |