Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTT)4
Start:614607 End:614618
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTGCCCCAAGAAATGATTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGATACCTACCCTTTTCGC | |||||||||||||
| Tm_F_1 | 60.014 | Size_F_1 | 20 | Tm_R_1 | 59.799 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 614499 | Primer End | 614731 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACACATCTGAGATCGCCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGCAGAACCCCCTACCCTA | |||||||||||||
| Tm_F_2 | 59.794 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 614551 | Primer End | 614830 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTGCCCCAAGAAATGATTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTACCCTTTTCGCAACTCA | |||||||||||||
| Tm_F_3 | 60.014 | Size_F_3 | 20 | Tm_R_3 | 60.241 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 614499 | Primer End | 614725 |