Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTCA)3
Start:349016 End:349027
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCAAGCTGACGTGACGAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTCTGTCTTGCTTGGTTGC | |||||||||||||
| Tm_F_1 | 59.622 | Size_F_1 | 20 | Tm_R_1 | 60.032 | Size_R_1 | 20 | PRODUCT Size | 198 bp | Primer Start | 348884 | Primer End | 349081 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGTGACGAGAAAGCCAAAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTGCTCTTCTTGGTTGCT | |||||||||||||
| Tm_F_2 | 60.299 | Size_F_2 | 20 | Tm_R_2 | 59.763 | Size_R_2 | 20 | PRODUCT Size | 109 bp | Primer Start | 348955 | Primer End | 349063 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGCTGACGTGACGAACACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTCTGTCTTGCTTGGTTGC | |||||||||||||
| Tm_F_3 | 60.51 | Size_F_3 | 20 | Tm_R_3 | 60.032 | Size_R_3 | 20 | PRODUCT Size | 195 bp | Primer Start | 348887 | Primer End | 349081 |