Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CTAA)3atcatgataattccttgtttacgaggt(GAAA)3gact(GAAA)4
Start:674643 End:674713
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACATGCTGAGGGGAGAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGGCTTGGCTGATCTTAC | |||||||||||||
| Tm_F_1 | 59.803 | Size_F_1 | 20 | Tm_R_1 | 59.978 | Size_R_1 | 20 | PRODUCT Size | 211 bp | Primer Start | 674558 | Primer End | 674768 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACATGCTGAGGGGAGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTCGGCTTGGCTGATCTTA | |||||||||||||
| Tm_F_2 | 59.803 | Size_F_2 | 20 | Tm_R_2 | 59.978 | Size_R_2 | 20 | PRODUCT Size | 212 bp | Primer Start | 674558 | Primer End | 674769 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGGGTGCTTCATCTCAGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCGGCTTGGCTGATCTTAC | |||||||||||||
| Tm_F_3 | 60.261 | Size_F_3 | 20 | Tm_R_3 | 59.978 | Size_R_3 | 20 | PRODUCT Size | 241 bp | Primer Start | 674528 | Primer End | 674768 |