Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CT)7gtctacttgcttggcttgcttgttctccctccttcccttgcttgcgcttcctgcgtaaca(GCTT)3
Start:543729 End:543814
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGTAACTAACTGCTTGCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGCAGGAGCAGACATACAGA | |||||||||||||
| Tm_F_1 | 59.911 | Size_F_1 | 20 | Tm_R_1 | 60.16 | Size_R_1 | 20 | PRODUCT Size | 165 bp | Primer Start | 543696 | Primer End | 543860 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCGTAACTAACTGCTTGCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGAGCTAAGATTCCCCAGCC | |||||||||||||
| Tm_F_2 | 59.911 | Size_F_2 | 20 | Tm_R_2 | 59.811 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 543696 | Primer End | 543935 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCGTAACTAACTGCTTGCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGTTACGCAGGAGCAGACA | |||||||||||||
| Tm_F_3 | 59.911 | Size_F_3 | 20 | Tm_R_3 | 60.199 | Size_R_3 | 20 | PRODUCT Size | 171 bp | Primer Start | 543696 | Primer End | 543866 |