Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CGGC)3tt(CTTG)4
Start:916333 End:916362
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTTCCCGCTTTGACTCTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGAAGGCAATGAAATTGTGA | |||||||||||||
| Tm_F_1 | 59.948 | Size_F_1 | 20 | Tm_R_1 | 59.664 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 916182 | Primer End | 916452 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGACCTCACTCCTCTCCCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAATTAAAGTAAGCCGGGG | |||||||||||||
| Tm_F_2 | 60.198 | Size_F_2 | 20 | Tm_R_2 | 59.802 | Size_R_2 | 20 | PRODUCT Size | 222 bp | Primer Start | 916278 | Primer End | 916499 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGACCTCACTCCTCTCCCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGAATTAAAGTAAGCCGGG | |||||||||||||
| Tm_F_3 | 60.198 | Size_F_3 | 20 | Tm_R_3 | 59.802 | Size_R_3 | 20 | PRODUCT Size | 223 bp | Primer Start | 916278 | Primer End | 916500 |