Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CCTG)4
Start:407257 End:407272
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATTCCCAAGGTAAAAGCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGAAATCAGGGGAAGGACA | |||||||||||||
| Tm_F_1 | 59.931 | Size_F_1 | 20 | Tm_R_1 | 60.042 | Size_R_1 | 20 | PRODUCT Size | 141 bp | Primer Start | 407220 | Primer End | 407360 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATTCCCAAGGTAAAAGCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAAAGAAAGAGGAGCGCAGC | |||||||||||||
| Tm_F_2 | 59.931 | Size_F_2 | 20 | Tm_R_2 | 59.867 | Size_R_2 | 20 | PRODUCT Size | 229 bp | Primer Start | 407220 | Primer End | 407448 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATTCCCAAGGTAAAAGCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTAATCATCCCTCCCCCTA | |||||||||||||
| Tm_F_3 | 59.931 | Size_F_3 | 20 | Tm_R_3 | 60.245 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 407220 | Primer End | 407485 |