Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CCAG)3
Start:460613 End:460624
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGAAAAGGGTCAGGCTCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGGGGTAAAATGGAATGGA | |||||||||||||
| Tm_F_1 | 60.074 | Size_F_1 | 20 | Tm_R_1 | 59.988 | Size_R_1 | 20 | PRODUCT Size | 216 bp | Primer Start | 460562 | Primer End | 460777 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTCAGGCATGAGACCGAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCACCGAAGAGGAACTGTA | |||||||||||||
| Tm_F_2 | 60.269 | Size_F_2 | 20 | Tm_R_2 | 60.103 | Size_R_2 | 20 | PRODUCT Size | 207 bp | Primer Start | 460442 | Primer End | 460648 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGAAAAGGGTCAGGCTCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTTCCGAGGAATTCTCCTT | |||||||||||||
| Tm_F_3 | 60.074 | Size_F_3 | 20 | Tm_R_3 | 59.646 | Size_R_3 | 20 | PRODUCT Size | 117 bp | Primer Start | 460562 | Primer End | 460678 |