Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CCAA)3
Start:829570 End:829581
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTAAGGGCATATCGGTTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGAAGGAACGGGAAGACCT | |||||||||||||
| Tm_F_1 | 60.06 | Size_F_1 | 20 | Tm_R_1 | 59.935 | Size_R_1 | 20 | PRODUCT Size | 260 bp | Primer Start | 829384 | Primer End | 829643 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCATACGCCTTTCTGGATGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGAAGGAACGGGAAGACCT | |||||||||||||
| Tm_F_2 | 60.103 | Size_F_2 | 20 | Tm_R_2 | 59.935 | Size_R_2 | 20 | PRODUCT Size | 186 bp | Primer Start | 829458 | Primer End | 829643 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGCATACGCCTTTCTGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGAAGGAACGGGAAGACCT | |||||||||||||
| Tm_F_3 | 59.845 | Size_F_3 | 20 | Tm_R_3 | 59.935 | Size_R_3 | 20 | PRODUCT Size | 189 bp | Primer Start | 829455 | Primer End | 829643 |