Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CAT)4
Start:618900 End:618911
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGAAAGGCCTCTAAACGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAACTCGCAAAGACTCGACC | |||||||||||||
| Tm_F_1 | 59.953 | Size_F_1 | 20 | Tm_R_1 | 59.997 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 618820 | Primer End | 619099 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATGCTTGTGATTCCACACCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGCTCGTTTGGCTGACTTT | |||||||||||||
| Tm_F_2 | 59.967 | Size_F_2 | 20 | Tm_R_2 | 59.621 | Size_R_2 | 20 | PRODUCT Size | 215 bp | Primer Start | 618776 | Primer End | 618990 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGCTTGTGATTCCACACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGTTTGAGCTCGTTTGGCT | |||||||||||||
| Tm_F_3 | 59.967 | Size_F_3 | 20 | Tm_R_3 | 59.621 | Size_R_3 | 20 | PRODUCT Size | 221 bp | Primer Start | 618776 | Primer End | 618996 |