Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CAGC)3
Start:144515 End:144526
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGATCCCCACAATAGCTGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTGCTAAATAGAGCGCCCA | |||||||||||||
| Tm_F_1 | 59.894 | Size_F_1 | 20 | Tm_R_1 | 59.838 | Size_R_1 | 20 | PRODUCT Size | 249 bp | Primer Start | 144413 | Primer End | 144661 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGATCCCCACAATAGCTGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCCAGGCCTTCCAACCTAT | |||||||||||||
| Tm_F_2 | 59.894 | Size_F_2 | 20 | Tm_R_2 | 59.789 | Size_R_2 | 20 | PRODUCT Size | 208 bp | Primer Start | 144413 | Primer End | 144620 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGATCCCCACAATAGCTGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCAGGCCTTCCAACCTATTT | |||||||||||||
| Tm_F_3 | 59.894 | Size_F_3 | 20 | Tm_R_3 | 60.312 | Size_R_3 | 20 | PRODUCT Size | 206 bp | Primer Start | 144413 | Primer End | 144618 |