Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(CAGA)3
Start:963119 End:963130
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTTCACTTTCTTTGCTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGCCGGATCAACTGACAAC | |||||||||||||
| Tm_F_1 | 59.846 | Size_F_1 | 20 | Tm_R_1 | 60.119 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 962912 | Primer End | 963191 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCAAATCCCTTCGCAATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGCCGGATCAACTGACAAC | |||||||||||||
| Tm_F_2 | 60.155 | Size_F_2 | 20 | Tm_R_2 | 60.119 | Size_R_2 | 20 | PRODUCT Size | 177 bp | Primer Start | 963015 | Primer End | 963191 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTCAAATCCCTTCGCAATC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAGAAATAAGCCACTGCCCG | |||||||||||||
| Tm_F_3 | 60.155 | Size_F_3 | 20 | Tm_R_3 | 60.223 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 963015 | Primer End | 963291 |