Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ATTT)3
Start:592600 End:592611
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACAGAACTCCGGCTCAATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGTTTACTGGGAATAGCGG | |||||||||||||
| Tm_F_1 | 59.81 | Size_F_1 | 20 | Tm_R_1 | 60.089 | Size_R_1 | 20 | PRODUCT Size | 217 bp | Primer Start | 592538 | Primer End | 592754 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAATGAGACAGAACTCCGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGTTTACTGGGAATAGCGG | |||||||||||||
| Tm_F_2 | 59.81 | Size_F_2 | 20 | Tm_R_2 | 60.089 | Size_R_2 | 20 | PRODUCT Size | 223 bp | Primer Start | 592532 | Primer End | 592754 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACCCGATTGAACGAGCTTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGGAAGGTTCCGGAGTTCT | |||||||||||||
| Tm_F_3 | 59.708 | Size_F_3 | 20 | Tm_R_3 | 59.935 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 592447 | Primer End | 592726 |