Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ATTA)3gttgcttaagtagagtaagtaaatggcgtaaccgtaaccgaacactctctttgtttgtttcattgaaggtaggtagagtaaag(ATTC)3
Start:538175 End:538281
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCTTTTCTTTCTGCTGCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTCGCCCTTCTTTACTGTG | |||||||||||||
| Tm_F_1 | 58.39 | Size_F_1 | 21 | Tm_R_1 | 60.015 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 538126 | Primer End | 538395 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCCTTTTCTTTCTGCTGCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGGCTCGCCCTTCTTTACT | |||||||||||||
| Tm_F_2 | 58.39 | Size_F_2 | 21 | Tm_R_2 | 59.983 | Size_R_2 | 20 | PRODUCT Size | 273 bp | Primer Start | 538126 | Primer End | 538398 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTCCTTTTCTTTCTGCTGCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTCGCCCTTCTTTACTGTG | |||||||||||||
| Tm_F_3 | 59.326 | Size_F_3 | 22 | Tm_R_3 | 60.015 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 538125 | Primer End | 538395 |