Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ATTA)3
Start:423898 End:423909
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGGAGGTCAAGCAGCTAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCCAACCCAGCCTATTTCC | |||||||||||||
| Tm_F_1 | 59.978 | Size_F_1 | 20 | Tm_R_1 | 60.152 | Size_R_1 | 20 | PRODUCT Size | 194 bp | Primer Start | 423782 | Primer End | 423975 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGGAGGTCAAGCAGCTAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTGGGCAAGAGTGAAAGA | |||||||||||||
| Tm_F_2 | 59.978 | Size_F_2 | 20 | Tm_R_2 | 60.517 | Size_R_2 | 20 | PRODUCT Size | 171 bp | Primer Start | 423782 | Primer End | 423952 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCTTGCTTTCTTTTCCTCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCCAACCCAGCCTATTTCC | |||||||||||||
| Tm_F_3 | 59.564 | Size_F_3 | 20 | Tm_R_3 | 60.152 | Size_R_3 | 20 | PRODUCT Size | 211 bp | Primer Start | 423765 | Primer End | 423975 |