Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ATCA)3
Start:291060 End:291071
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGCTGAAGGAGCGGATCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTTAACCTGAACCGGATGGA | |||||||||||||
| Tm_F_1 | 59.939 | Size_F_1 | 20 | Tm_R_1 | 59.926 | Size_R_1 | 20 | PRODUCT Size | 149 bp | Primer Start | 290991 | Primer End | 291139 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGGGAGGAAGTAGCTCGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTAACCTGAACCGGATGGA | |||||||||||||
| Tm_F_2 | 59.84 | Size_F_2 | 20 | Tm_R_2 | 59.926 | Size_R_2 | 20 | PRODUCT Size | 253 bp | Primer Start | 290887 | Primer End | 291139 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAACCACTGACCCCTTTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGAAAGCCTTCCCCTTCAC | |||||||||||||
| Tm_F_3 | 59.943 | Size_F_3 | 20 | Tm_R_3 | 60.19 | Size_R_3 | 20 | PRODUCT Size | 262 bp | Primer Start | 290841 | Primer End | 291102 |