Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ATAGT)3
Start:398054 End:398068
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACTGACAGCTTTCGTTGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATTTAAGGGGGAGGGAGTC | |||||||||||||
| Tm_F_1 | 60.44 | Size_F_1 | 20 | Tm_R_1 | 59.761 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 397924 | Primer End | 398173 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGCAGGGAGTCCTTTCTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATTTAAGGGGGAGGGAGTC | |||||||||||||
| Tm_F_2 | 60.523 | Size_F_2 | 20 | Tm_R_2 | 59.761 | Size_R_2 | 20 | PRODUCT Size | 230 bp | Primer Start | 397944 | Primer End | 398173 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGAGAGAGGGGTGAAACCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGCCTCGATTCTATTCGTA | |||||||||||||
| Tm_F_3 | 60.619 | Size_F_3 | 20 | Tm_R_3 | 60.188 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 397996 | Primer End | 398230 |