Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ATA)5
Start:479976 End:479990
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAAGTTCAGTGGGGAAGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTAATCTATTCCCGCACGC | |||||||||||||
| Tm_F_1 | 60.476 | Size_F_1 | 20 | Tm_R_1 | 60.061 | Size_R_1 | 20 | PRODUCT Size | 244 bp | Primer Start | 479924 | Primer End | 480167 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGAAAGGGAATCCAGTGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGATTCGTCAGACTTGAGC | |||||||||||||
| Tm_F_2 | 60.05 | Size_F_2 | 20 | Tm_R_2 | 60.945 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 479821 | Primer End | 480087 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGAAAGGGAATCCAGTGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GACGGATTCGTCAGACTTGAG | |||||||||||||
| Tm_F_3 | 60.05 | Size_F_3 | 20 | Tm_R_3 | 59.859 | Size_R_3 | 21 | PRODUCT Size | 269 bp | Primer Start | 479821 | Primer End | 480089 |