Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGTCT)3
Start:492016 End:492030
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTTGTCCGTGGGTAGAAGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAAAGAGCAGGAGCTTCGTT | |||||||||||||
| Tm_F_1 | 59.966 | Size_F_1 | 20 | Tm_R_1 | 59.757 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 491992 | Primer End | 492270 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTGTCCGTGGGTAGAAGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAGCAGTTTTGGATTGCCTT | |||||||||||||
| Tm_F_2 | 59.966 | Size_F_2 | 20 | Tm_R_2 | 60.249 | Size_R_2 | 20 | PRODUCT Size | 178 bp | Primer Start | 491992 | Primer End | 492169 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAGAAAGGAACCGAGCTGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGACAACTCGACAACGGAC | |||||||||||||
| Tm_F_3 | 59.989 | Size_F_3 | 20 | Tm_R_3 | 59.726 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 491877 | Primer End | 492125 |