Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGTC)3
Start:696156 End:696167
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGAATGCGCCGCTAACTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCGTGCTAATCTTGGTATGGA | |||||||||||||
| Tm_F_1 | 59.732 | Size_F_1 | 20 | Tm_R_1 | 59.967 | Size_R_1 | 21 | PRODUCT Size | 272 bp | Primer Start | 696025 | Primer End | 696296 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACCTTGATACGGAGCTGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCATGAACGTAATGCTCACAA | |||||||||||||
| Tm_F_2 | 60.218 | Size_F_2 | 20 | Tm_R_2 | 59.75 | Size_R_2 | 21 | PRODUCT Size | 280 bp | Primer Start | 695990 | Primer End | 696269 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGAATGCGCCGCTAACTAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCATGAACGTAATGCTCACAA | |||||||||||||
| Tm_F_3 | 59.732 | Size_F_3 | 20 | Tm_R_3 | 59.75 | Size_R_3 | 21 | PRODUCT Size | 245 bp | Primer Start | 696025 | Primer End | 696269 |