Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGTAGG)3
Start:777236 End:777253
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCTTTCCTCTCCCGGATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCGGAGCTACTTCTCCTTT | |||||||||||||
| Tm_F_1 | 60.147 | Size_F_1 | 20 | Tm_R_1 | 59.842 | Size_R_1 | 20 | PRODUCT Size | 238 bp | Primer Start | 777089 | Primer End | 777326 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGAATAGAAGGTCAAGGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCGGAGCTACTTCTCCTTT | |||||||||||||
| Tm_F_2 | 59.761 | Size_F_2 | 20 | Tm_R_2 | 59.842 | Size_R_2 | 20 | PRODUCT Size | 202 bp | Primer Start | 777125 | Primer End | 777326 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGAATAGAAGGTCAAGGGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTCCCGGAGCTACTTCTCC | |||||||||||||
| Tm_F_3 | 59.903 | Size_F_3 | 20 | Tm_R_3 | 59.674 | Size_R_3 | 20 | PRODUCT Size | 204 bp | Primer Start | 777126 | Primer End | 777329 |