Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGTA)4
Start:542137 End:542152
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCATTGCCTTCTTTTCGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCCTTACGGTCAGCAGCTA | |||||||||||||
| Tm_F_1 | 59.685 | Size_F_1 | 20 | Tm_R_1 | 58.283 | Size_R_1 | 20 | PRODUCT Size | 176 bp | Primer Start | 542051 | Primer End | 542226 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTCTCACAATTCCATTGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCCTTACGGTCAGCAGCTA | |||||||||||||
| Tm_F_2 | 60.326 | Size_F_2 | 20 | Tm_R_2 | 58.283 | Size_R_2 | 20 | PRODUCT Size | 186 bp | Primer Start | 542041 | Primer End | 542226 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGCGGCTGAACCTGATACT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCCTTACGGTCAGCAGCTA | |||||||||||||
| Tm_F_3 | 60.419 | Size_F_3 | 20 | Tm_R_3 | 58.283 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 541964 | Primer End | 542226 |