Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGTA)3
Start:723602 End:723613
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGCGAAGACTGGAAAGGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTCAGCCACTTTGACCCAAT | |||||||||||||
| Tm_F_1 | 60.126 | Size_F_1 | 20 | Tm_R_1 | 59.973 | Size_R_1 | 20 | PRODUCT Size | 257 bp | Primer Start | 723387 | Primer End | 723643 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATGGATCAACAACATTGCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTCAGCCACTTTGACCCAAT | |||||||||||||
| Tm_F_2 | 59.781 | Size_F_2 | 20 | Tm_R_2 | 59.973 | Size_R_2 | 20 | PRODUCT Size | 166 bp | Primer Start | 723478 | Primer End | 723643 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGCAGCGAAGACTGGAAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCAGCCACTTTGACCCAAT | |||||||||||||
| Tm_F_3 | 59.757 | Size_F_3 | 20 | Tm_R_3 | 59.973 | Size_R_3 | 20 | PRODUCT Size | 260 bp | Primer Start | 723384 | Primer End | 723643 |