Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGTA)3
Start:712505 End:712516
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGAATGAGTGAAGTTCGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTGGCTGCATCTTCTGTCC | |||||||||||||
| Tm_F_1 | 60.05 | Size_F_1 | 20 | Tm_R_1 | 60.226 | Size_R_1 | 20 | PRODUCT Size | 177 bp | Primer Start | 712382 | Primer End | 712558 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCAAGCAAACAGACCGAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTGGCTGCATCTTCTGTCC | |||||||||||||
| Tm_F_2 | 60.058 | Size_F_2 | 20 | Tm_R_2 | 60.226 | Size_R_2 | 20 | PRODUCT Size | 107 bp | Primer Start | 712452 | Primer End | 712558 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGAATGAGTGAAGTTCGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGGCTATTGGCTGCATCTT | |||||||||||||
| Tm_F_3 | 60.05 | Size_F_3 | 20 | Tm_R_3 | 60.374 | Size_R_3 | 20 | PRODUCT Size | 183 bp | Primer Start | 712382 | Primer End | 712564 |