Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGGCT)3ggctttcactc(CTTG)3ctgggtgtt(GGAA)3
Start:388474 End:388532
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCCTGTCTGTCCCCCTTAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTTTAGGTGAGTGCCTGCC | |||||||||||||
| Tm_F_1 | 60.23 | Size_F_1 | 20 | Tm_R_1 | 60.397 | Size_R_1 | 20 | PRODUCT Size | 179 bp | Primer Start | 388381 | Primer End | 388559 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTTTACGGGACAGGCTAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTTAGGTGAGTGCCTGCC | |||||||||||||
| Tm_F_2 | 59.763 | Size_F_2 | 20 | Tm_R_2 | 60.397 | Size_R_2 | 20 | PRODUCT Size | 109 bp | Primer Start | 388451 | Primer End | 388559 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATACCCTGTCTGTCCCCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTTTAGGTGAGTGCCTGCC | |||||||||||||
| Tm_F_3 | 59.676 | Size_F_3 | 20 | Tm_R_3 | 60.397 | Size_R_3 | 20 | PRODUCT Size | 181 bp | Primer Start | 388379 | Primer End | 388559 |