Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGGAG)3
Start:816149 End:816163
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAGACCGAGTTTACGAGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCCTCATTCTCCCTTCAA | |||||||||||||
| Tm_F_1 | 60.397 | Size_F_1 | 20 | Tm_R_1 | 59.6 | Size_R_1 | 20 | PRODUCT Size | 248 bp | Primer Start | 816019 | Primer End | 816266 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTTTACGAGGCGAACAAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCCCTCATTCTCCCTTCAA | |||||||||||||
| Tm_F_2 | 59.522 | Size_F_2 | 20 | Tm_R_2 | 59.6 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 816027 | Primer End | 816266 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGGAAAGGAGTGAAAGGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCTTGTGGTCTCATTGCAT | |||||||||||||
| Tm_F_3 | 60.045 | Size_F_3 | 20 | Tm_R_3 | 59.09 | Size_R_3 | 20 | PRODUCT Size | 126 bp | Primer Start | 816064 | Primer End | 816189 |