Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGGAC)3
Start:887484 End:887498
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCGGGTCAGGTTCAATAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGGGGTCAAAATCGAATA | |||||||||||||
| Tm_F_1 | 59.926 | Size_F_1 | 20 | Tm_R_1 | 59.894 | Size_R_1 | 20 | PRODUCT Size | 180 bp | Primer Start | 887355 | Primer End | 887534 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCCGGGTCAGGTTCAATAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAAGGAAAGAAGGGAATGG | |||||||||||||
| Tm_F_2 | 59.926 | Size_F_2 | 20 | Tm_R_2 | 59.875 | Size_R_2 | 20 | PRODUCT Size | 271 bp | Primer Start | 887355 | Primer End | 887625 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAGGCAGGGTTACACTCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCGGGGTCAAAATCGAATA | |||||||||||||
| Tm_F_3 | 59.874 | Size_F_3 | 20 | Tm_R_3 | 59.894 | Size_R_3 | 20 | PRODUCT Size | 139 bp | Primer Start | 887396 | Primer End | 887534 |