Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGCT)3
Start:645209 End:645220
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGAGTTCAAGAAGTCCAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAAGCAGTTTAGAGCCGGAG | |||||||||||||
| Tm_F_1 | 59.837 | Size_F_1 | 20 | Tm_R_1 | 60.147 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 645139 | Primer End | 645408 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGAGTTCAAGAAGTCCAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTGAAGGAGTGAACGAAGC | |||||||||||||
| Tm_F_2 | 59.837 | Size_F_2 | 20 | Tm_R_2 | 59.851 | Size_R_2 | 20 | PRODUCT Size | 232 bp | Primer Start | 645139 | Primer End | 645370 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATCAGTCCACTGGAGGGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAAGGAGTGAACGAAGCAA | |||||||||||||
| Tm_F_3 | 59.962 | Size_F_3 | 20 | Tm_R_3 | 59.566 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 645089 | Primer End | 645368 |