Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGCT)3
Start:534682 End:534693
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTTGGAAGGACCAAGTCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTCAGGGAATGAATATCGC | |||||||||||||
| Tm_F_1 | 59.943 | Size_F_1 | 20 | Tm_R_1 | 60.435 | Size_R_1 | 20 | PRODUCT Size | 180 bp | Primer Start | 534649 | Primer End | 534828 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTTGGAAGGACCAAGTCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGTTCACGTCAGGGAATGA | |||||||||||||
| Tm_F_2 | 59.943 | Size_F_2 | 20 | Tm_R_2 | 60.51 | Size_R_2 | 20 | PRODUCT Size | 187 bp | Primer Start | 534649 | Primer End | 534835 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTCTTCTTGCTTGCTGGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTCAGGGAATGAATATCGC | |||||||||||||
| Tm_F_3 | 60.134 | Size_F_3 | 20 | Tm_R_3 | 60.435 | Size_R_3 | 20 | PRODUCT Size | 197 bp | Primer Start | 534632 | Primer End | 534828 |