Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGCA)3
Start:462458 End:462469
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGAAGACCTGAGACCCGTAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTCTTTCTTGCTGACTGGG | |||||||||||||
| Tm_F_1 | 59.861 | Size_F_1 | 20 | Tm_R_1 | 59.982 | Size_R_1 | 20 | PRODUCT Size | 121 bp | Primer Start | 462388 | Primer End | 462508 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAGAAGCTCGACTGGAAAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTCCTGGTTGGCCTATGAC | |||||||||||||
| Tm_F_2 | 60.126 | Size_F_2 | 20 | Tm_R_2 | 60.073 | Size_R_2 | 20 | PRODUCT Size | 141 bp | Primer Start | 462420 | Primer End | 462560 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGAAGACCTGAGACCCGTAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTCCTGGTTGGCCTATGAC | |||||||||||||
| Tm_F_3 | 59.861 | Size_F_3 | 20 | Tm_R_3 | 60.073 | Size_R_3 | 20 | PRODUCT Size | 173 bp | Primer Start | 462388 | Primer End | 462560 |