Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAT)3
Start:602506 End:602517
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCAGGACCATTCCTCAAAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAAGGGACAGGAGAGAAGCC | |||||||||||||
| Tm_F_1 | 60.042 | Size_F_1 | 20 | Tm_R_1 | 60.336 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 602323 | Primer End | 602555 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCCGTCACCTCTATTCGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTCTGGTTCGGGTTACAGG | |||||||||||||
| Tm_F_2 | 60.362 | Size_F_2 | 20 | Tm_R_2 | 60.103 | Size_R_2 | 20 | PRODUCT Size | 198 bp | Primer Start | 602449 | Primer End | 602646 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAATGAAGCGAAAACCAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAAGGGACAGGAGAGAAGCC | |||||||||||||
| Tm_F_3 | 60.192 | Size_F_3 | 20 | Tm_R_3 | 60.336 | Size_R_3 | 20 | PRODUCT Size | 247 bp | Primer Start | 602309 | Primer End | 602555 |