Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAGA)3
Start:112099 End:112113
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTAGGCGGCAGTTGAAAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGCTGAAGCTATCGTGTCG | |||||||||||||
| Tm_F_1 | 59.845 | Size_F_1 | 20 | Tm_R_1 | 60.157 | Size_R_1 | 20 | PRODUCT Size | 252 bp | Primer Start | 111920 | Primer End | 112171 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTAGGCGGCAGTTGAAAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTCGTATGGATGTGGATGG | |||||||||||||
| Tm_F_2 | 59.845 | Size_F_2 | 20 | Tm_R_2 | 60.201 | Size_R_2 | 20 | PRODUCT Size | 237 bp | Primer Start | 111920 | Primer End | 112156 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAACCACGAGCAATTCGTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGAGCGAAACCACCTTCAAA | |||||||||||||
| Tm_F_3 | 59.74 | Size_F_3 | 20 | Tm_R_3 | 59.853 | Size_R_3 | 20 | PRODUCT Size | 272 bp | Primer Start | 112034 | Primer End | 112305 |