Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAAA)4
Start:163276 End:163295
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAATGAAAACGTGACGGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGGCTCTAAACAGCTTGGT | |||||||||||||
| Tm_F_1 | 60.088 | Size_F_1 | 20 | Tm_R_1 | 60.015 | Size_R_1 | 20 | PRODUCT Size | 235 bp | Primer Start | 163118 | Primer End | 163352 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAATGAAAACGTGACGGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTAAAGCTCCTTCGGCTCT | |||||||||||||
| Tm_F_2 | 60.088 | Size_F_2 | 20 | Tm_R_2 | 59.983 | Size_R_2 | 20 | PRODUCT Size | 247 bp | Primer Start | 163118 | Primer End | 163364 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAAAACGTGACGGAATTGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGGCTCTAAACAGCTTGGT | |||||||||||||
| Tm_F_3 | 59.836 | Size_F_3 | 20 | Tm_R_3 | 60.015 | Size_R_3 | 20 | PRODUCT Size | 230 bp | Primer Start | 163123 | Primer End | 163352 |