Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAA)5
Start:55027 End:55046
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGAAGATCAGCTGCGAGACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCATTTGATTCCCCTTCA | |||||||||||||
| Tm_F_1 | 59.709 | Size_F_1 | 20 | Tm_R_1 | 60.244 | Size_R_1 | 20 | PRODUCT Size | 168 bp | Primer Start | 54996 | Primer End | 55163 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGAGAGGGGAGAAGATCAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCCATTTGATTCCCCTTCA | |||||||||||||
| Tm_F_2 | 60.3 | Size_F_2 | 20 | Tm_R_2 | 60.244 | Size_R_2 | 20 | PRODUCT Size | 177 bp | Primer Start | 54987 | Primer End | 55163 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGAAGATCAGCTGCGAGACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCATTTGATTCCCCTTCAC | |||||||||||||
| Tm_F_3 | 59.709 | Size_F_3 | 20 | Tm_R_3 | 59.727 | Size_R_3 | 20 | PRODUCT Size | 167 bp | Primer Start | 54996 | Primer End | 55162 |