Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAA)3aaagaaagaggctttccacatatcatatgtattgtcctaaggatttgtatcagctgtagc(AAAG)3
Start:293567 End:293650
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACTAAACCCCACCCCTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTTTTGATTGTATCGGCCA | |||||||||||||
| Tm_F_1 | 59.668 | Size_F_1 | 20 | Tm_R_1 | 59.93 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 293506 | Primer End | 293778 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGCGCACTAGAACTAAACCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCCAAAACCCTGACTAATG | |||||||||||||
| Tm_F_2 | 59.904 | Size_F_2 | 20 | Tm_R_2 | 59.429 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 293497 | Primer End | 293763 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCGCACTAGAACTAAACCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCAAAACCCTGACTAATGG | |||||||||||||
| Tm_F_3 | 59.904 | Size_F_3 | 20 | Tm_R_3 | 59.429 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 293497 | Primer End | 293762 |